ABSTRACT There are several products that have been used for the control of Sitophilus oryzae L. However, the high resistance of the insect to various chemical products has motivated the search for new alternatives for its control, which led to evaluating the in vitro effect of the extracts from Pimenta dioica (L.) Merr, Callistemon citrinus (Curtis) Skeels and Syzygium malaccense (L.) Merr. & L.M. Perry on S. oryzae. The plant material was ground into particles smaller than 1 mm. The powder obtained was used to obtain extracts through three extraction methods (Soxhlet, assisted extraction with Ultrasound and Maceration), with n-hexane, ethyl acetate and methanol solvents. The yield of the extracts was determined and the biological action on S. oryzae was evaluated by means of the percentage of insect emergency mortality and weight loss. The extracts formed by Soxhlet and n-hexane were more effective in vitro on S. oryzae, but all showed high repellency.

Resumen Son varios los productos que han sido utilizados para el control de Sitophilus oryzae L.; sin embargo, la alta resistencia del insecto a diversos productos químicos ha motivado la búsqueda de nuevas alternativas para su control, lo que motivó a evaluar el efecto in vitro de los extractos de Pimenta dioica (L.) Merr, Callistemon citrinus (Curtis) Skeels y Syzygium malaccense (L.) Merr. &L.M. Perry sobre S. oryzae. El material vegetal fue molinado en partículas menores de 1 mm. El polvo obtenido fue utilizado para la obtención de extractos a través de tres métodos de extracción (Soxhlet, extracción asistida con Ultrasonido y Maceración), con solventes n-hexano, acetato de etilo y metanol. Se determinó el rendimiento de los extractos y fue evaluada la acción biológica sobre S. oryzae mediante el porcentaje de mortalidad emergencia del insecto y pérdida de peso. Los extractos conformados por Soxhlet, n-hexano fueron más efectivos en el control in vitro de S. oryzae, pero todos los extractos mostraron alta repelencia.

Resumo: Sementes de azevém com baixa qualidade fisiológica têm resultado em pastagens com inadequado estande de plantas para a cobertura do solo e, sobretudo, para a exploração forrageira. Neste sentido, o presente trabalho teve como objetivo propor um teste de vigor para sementes de azevém anual. Foram testadas profundidades de semeadura (0,5; 1,0; 1,5; 2,0; 2,5 e 3 cm) em três substratos (areia, fibra de coco e casca de arroz carbonizada) para diferenciar a qualidade fisiológica de quatro lotes de sementes da cultivar BRS Ponteio que apresentaram semelhança no teste de germinação e umidade. Os lotes foram classificados quanto ao vigor pelos testes de emergência em campo, peso de mil sementes, envelhecimento acelerado e primeira contagem da germinação. A profundidade de semeadura em substratos de areia e fibra de coco permitiu a determinação do vigor em sementes de azevém anual (de maneira similar ao observado no teste de germinação no campo), pela porcentagem de emergência e pelo índice de velocidade de emergência de plântulas semeadas em areia a 2,5 e em fibra de coco a 3,0 cm de profundidade de semeadura.

Abstract: Annual ryegrass seeds having low physiological quality can lead to the formation of pastures with undesirable plant stands for soil cover and forage utilization pastures. Thus, the objective of this study was proposing a vigor test to classify annual ryegrass seeds. For that, different sowing depths (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 cm) over three substrates (sand, coconut fiber, and carbonized rice husk) were tested to differentiate the physiological quality of four seed lots of annual ryegrass cv. BRS Ponteio having similarities in germination and moisture. The seed lots were classified according to vigor through the field seedling emergence (FSE), 1,000-seeds weight, saturated salt accelerated aging, and germination first count. The 2.5 cm of sand sowing depth and 3.0 cm of coconut fiber sowing depth provided separation of seed lots in similar groups of those reported to FSE test through the seedling emergence and speed of seedling emergence tests.

RESUMO CONTEXTO: A infecção pelo vírus da hepatite C (HCV) é uma das principais causas de hepatite C crônica e provoca implicações graves para pacientes, familiares e sistema de saúde. OBJETIVO: Os objetivos deste estudo foram: analisar a gravidade da fibrose hepática, comorbidades e complicações da hepatite C; examinar a qualidade de vida relacionada à saúde (QVRS), a perda de produtividade e o uso de recursos e custos no sistema público por pacientes brasileiros com hepatite C crônica, genótipo tipo 1. MÉTODOS: Foi realizado um estudo transversal, multicêntrico em pacientes com hepatite C crônica genótipo-1 para avaliar a carga da doença no sistema público de saúde brasileiro entre novembro de 2014 e março de 2015. Os pacientes foram submetidos a uma elastografia hepática transitória (FibroScan) para avaliar a fibrose e a uma entrevista composta por um questionário desenvolvido para o estudo e cinco questionários padronizados: EQ-5D-3L, HCV-PRO, e WPAI:HepC. RESULTADOS: Foram recrutados 313 pacientes. A amostra foi composta predominantemente por mulheres (50,8%), caucasianos/brancos (55,9%) e indivíduos empregados (39,9%). A média de idade foi 56 (DP=10,4) anos. Em média, os pacientes com HCV esperaram 40,6 (DP=49,6) meses entre o diagnóstico e o primeiro tratamento. Ademais, 42,8% dos pacientes que realizaram o FibroScan tinham cirrose; a comorbidade mais frequente foi doença cardiovascular (62,6%) e a complicação mais comum as varizes esofágicas (54,5%). Os resultados também mostraram que “dor e desconforto” foi a dimensão de QVRS mais afetada (55,0% dos pacientes relataram alguns problemas) e que a média do escore do HCV-PRO foi 69,1 (DP=24,2). Em relação à perda de produtividade, o componente do WPAI:HepC mais afetado foi atividade diária (23,5%) e entre os pacientes empregados, presenteísmo foi mais frequente do que absenteísmo (18,5% vs 6,5%). Os custos diretos médicos totais com essa amostra foi de 12.305,72USD por paciente em um período de dois anos; o tratamento medicamentoso representou 95% desse total. CONCLUSÃO Esse estudo mostrou a maioria dos pacientes possui cirrose, apresenta alta prevalência de doenças cardiometabolicas e varizes esofágicas, QVRS reduzida principalmente em termos de dor/desconforto e dano na produtividade, especialmente presenteísmo. Adicionalmente, nós demonstramos que o HCV impõe uma carga econômica no sistema de saúde brasileiro e que os medicamentos correspondem à maioria dos custos.

ABSTRACT BACKGROUND: Infection by hepatitis C virus is one of the leading causes of chronic hepatitis C and cause severe burden for patients, families and the health care system. OBJECTIVE: The aims of this research were to assess the severity of liver fibrosis, comorbidities and complications of hepatitis C virus; to examine health-related quality of life (HRQoL), productivity loss and resource use and costs in a sample of Brazilian chronic hepatitis C, genotype 1, patients. METHODS: This was a cross-sectional multicenter study performed in genotype-1 chronic hepatitis C patients to assess disease burden in the Brazilian public health care system between November 2014 and March 2015. Patients were submitted to a liver transient elastography (FibroScan) to assess liver fibrosis and answered an interview composed by a questionnaire specifically developed for the study and three standardized questionnaires: EQ-5D-3L, HCV-PRO and WPAI:HepC. RESULTS: There were 313 subjects enrolled, with predominance of women (50.8%), caucasian/white (55.9%) and employed individuals (39.9%). Mean age was 56 (SD=10.4) years old. Moreover, 42.8% of patients who underwent FibroScan were cirrhotic; the most frequent comorbidity was cardiovascular disease (62.6%) and the most frequent complication was esophageal varices (54.5%). The results also showed that “pain and discomfort” was the most affected HRQoL dimension (55.0% of patients reported some problems) and that the mean HCV-PRO overall score was 69.1 (SD=24.2). Regarding productivity loss, the most affected WPAI:HepC component was daily activity (23.5%) and among employed patients, presenteeism was more frequent than absenteeism (18.5% vs 6.5%). The direct medical costs in this chronic hepatitis C sample was 12,305.72USD per patient in the 2 years study period; drug treatment costs represented 95.9% of this total. CONCLUSION: This study showed that most patients are cirrhotic, present high prevalence of cardiometabolic diseases and esophageal varices, reduced HRQoL mainly in terms of pain/discomfort, and work productivity impairment, especially presenteeism. Additionally, we demonstrated that hepatitis C virus imposes an economic burden on Brazilian Health Care System and that most of this cost is due to drug treatment.

Abstract A 26-year-old postpartum female presented with symptoms characteristic of dengue fever on the 16th day of puerperium. On the third day of the illness, the patient presented a clinical picture consistent with shock. Tests determined primary infection with dengue virus serotype 2. Cardiac tamponade was confirmed by echocardiography. This rare manifestation is described in a patient without any associated comorbidity.

Colectinas e galectinas são proteínas da família das lectinas que possuem a capacidade de reconhecer padrões moleculares associados aos patógenos. Estudos em bovinos têm demonstrado a alta expressão dessas proteínas durante a infecção por nematoides gastrintestinais. O objetivo deste estudo foi investigar se o nível de infecção de Haemonchus contortus altera a expressão de colectinas (SPA e CGN) e galectinas (Gal11 e Gal14) de ovinos. Doze ovinos da raça Corriedale expostos a infecção natural com nematoides foram separados em dois grupos: grupo 1 (G1, n=7) com menor grau de parasitismo; e grupo 2 (G2, n=5) com maior grau, a partir da contagem do número de parasitos recuperados do abomaso e OPG. A contagem de OPG e de parasitos recuperados do abomaso dos grupos G1 e G2 apresentaram diferença estatística (p<0,05). A expressão dos genes de colectinas e galectina foi observada em todas as amostras de abomaso dos ovinos, porém animais com menor grau de infecção apresentaram menor expressão dos genes de Gal14, SPA e CGN (p<0,05). A expressão de lectinas foi associada ao número de H. contortus encontrados no abomaso de ovinos, indicando um possível papel dessas proteínas no controle da infecção.

Galectins and collectins are proteins classified in the lectin family that have the ability to recognize molecular patterns associated with pathogens. Studies on cattle have demonstrated high expression of these proteins during infection with gastrointestinal nematodes. The aim of this study was to investigate whether the level of Haemonchus contortus infection would alter the expression of galectins (Gal11 and Gal14) and collectins (SPA and CGN) in sheep. Twelve Corriedale sheep exposed to natural infection with nematodes were divided into two groups: group 1 (G1, n = 7) and group 2 (G2, n = 5), with low and high parasite burdens, respectively, based on fecal egg counts and abomasal parasite counts. The fecal egg counts and abomasal parasite counts were significantly different (p < 0.05) between the groups. Galectin and collectin gene expression was observed in all sheep abomasal samples. However, animals with lower infection levels showed lower expression of the genes Gal14, SPA and CGN (p < 0.05). Expression of lectins was associated with the abomasal H. contortus burden, thus suggesting that these proteins may have a role in controlling of this infection.

The objective of this study was to evaluate the chemical composition and fatty acid profile of meat from Santa Inês, Dorper × Santa Inês, and undefined-breed lambs finished in a feedlot system according to two forage:concentrate ratios (50:50 and 20:80) in the diet. Overall, 54 non-castrated animals were used (with an average initial weight of 23±1.5 kg at 150 days old) and were slaughtered when they reached 36 kg of live weight or 63 days of confinement. Diets with high concentrate levels favored meat production with higher protein content. Among the saturated fatty acids, only heptadecanoic (C17:0) and arachidic acids (C20:0) differed among the treatments. The amount of oleic acid detected in the meat ranged from 49.2 to 51.9 g/100 g, making this one the most representative fatty acids. Regardless of the diet and genotype, the unsaturated fatty acid and desirable fatty acid levels in the lamb meat characterize it as of satisfactory quality for the consumer market.

The bacterial isolates SUR - 6, DiP/2012 and Oct/Y were isolated from silkworms (Bombyx mori L.) infected naturally, which presented evident symptoms of bacterial flacheria. The goal of this study was to identify the bacterial pathogens associated with symptoms observed in silkworm breeding areas of Cuba, to establish control and sanitation measures. The morphological, biochemical and physiologic study allowed to identify these isolates as Enterobacter cloacae (SUR - 6), Serratia marcescens (DiP /2012), and Acinetobacter baumanii (Oct/Y), which are informed widely in the literature like pathogens of insects.

Los aislados bacterianos SUR - 6, DiP/2012 y Oct/Y se obtuvieron a partir de gusanos de seda (Bombyx mori L.) infectados naturalmente, los cuales presentaban síntomas evidentes de flacheria bacteriana. El presente trabajo tuvo como objetivo identificar los patógenos bacterianos asociados a estos síntomas observados en las crías de gusano de seda en zonas de Cuba, para establecer medidas de control y saneamiento. Los estudios morfológico, bioquímico y fisiológico de los aislados permitieron identificarlos como Enterobacter cloacae (SUR - 6), Serratia marcescens (DiP/2012) y Acinetobacter baumanii (Oct/Y), los cuales, en la literatura, son informados ampliamente como patógenos de insectos.

Salted lamb meat blanket, originated from boning, salting, and drying of whole lamb carcass, was studied aiming at obtaining information that support the search for guarantees of origin for this typical regional product from the city of Petrolina-Pernambuco-Brazil. Data from three processing units were obtained, where it was observed the use of a traditional local technology that uses salting, an ancient preservation method; however, with a peculiar boning technique, resulting in a meat product with great potential for exploitation in the form of meat blanket. Based on the values of pH (6.22 ± 0.22), water activity (0.97 ± 0.02), and moisture (69.86 ± 2.26) lamb meat blanket is considered a perishable product, and consequently it requires the use of other preservation methods combined with salt, which along with the results of the microbiological analyses (absence of Salmonella sp, score <10 MPN/g of halophilic bacteria, total coliforms between 6.7 × 10³ and 5.2 × 10(6) FUC/g, and Staphylococcus from 8.1 × 10³ CFU/g at uncountable) reinforce the need of hygienic practices to ensure product safety. These results, together with the product notoriety and the organization of the sector are important factors in achieving Geographical Indication of the Salted lamb Meat blanket of Petrolina.

Objectives: The aim of this study was to assess technical preferences of general dental practitioners when restoring anterior composite restorations. How the level of clinical experience or post-graduate training infuenced their options was also tested. Material and Methods: A cross-sectional study was performed using a questionnaire with general dental practitioners (GDPs) (n=276) in Southern Brazil. Information regarding post graduation training (specialization, master's or PhD degree) and linical experience (years since completing graduation) were gathered. The options regarding anterior composite restorations (type of composite, adhesive system, light curing unit, polishing procedures and rubber dam use) were collected. Data were submitted to descriptive analysis and associations were tested. Results: Response rate was 68% (187). GDPs selected microhybrid composite (52%) and 2-step total etch adhesive system (77%). LED was the preferred method of activation for 72.8%. Immediate polishing was preferred by 75%, using a combination of techniques. Most of the respondents (74.3%) did not use rubber dam. More experienced clinicians used more halogen lights (p<0.022), performed more light monitoring (p<0.001) and were resistant to use rubber dam (p<0.012). Dentists with post-graduation training used 3-etch-and-rinse system more frequently (p<0.04), usually monitored light intensity (p<0.014) and placed rubber dam more frequently (p<0.044). Conclusions: Hybrid composite, simplifed adhesives, LED units and immediate polishing were preferred by Southern Brazilian dentists for anterior composite restorations. Few dentists used rubber dam to perform composite restorations in anterior teeth. Clinical experience and post-graduation training infuenced the dentists' choices.

We present a case of ovarian metastasis secondary to a renal cell carcinoma, in a female patient aged 35 years old, assisted the consultation for abdominal tumor a year after being operated of a left renal cell carcinoma by videolaparoscopic surgery. In the studies made we confirmed a bilateral ovarian tumor. In the histopathologic study we confirmed the renal primary tumor as metastatic. The ovaries are common places of intrabdominal metastasis; around the 6 % of the ovarian cancer is secondary to primary tumors in the stomach, colon, mammas and lymphomas. The ovarian metastasis of a renal cell carcinoma is very infrequent.

Se presenta un caso de metástasis ovárica secundaria a un carcinoma de células renales, en una paciente femenina de 35 años de edad, que al año de haber sido operada de carcinoma de células renales izquierdo por cirugía videolaparoscopica, se consultó por tumoración abdominal. En los estudios realizados se confirma una tumoración de ovario bilateral. La intervención quirúrgica consistió en histerectomía total con doble enexectomía y omentectomía. En el estudio histopatológico se confirmó metastásico del tumor primario renal operado un año antes. Los ovarios son sitios comunes de metástasis intrabdominales, alrededor de un 6 % del cáncer de ovario es secundario a tumores primarios en estómago, colon, mamas y los linfomas. Las metástasis en ovario de un carcinoma de células renales son muy infrecuentes.

From january 2009 to december 2010, we carried out a descriptive observational study to identify the behavior of the patients of the Polyvalent Intensive Care Unit of the Teaching Military Hospital Mario Muñoz of Matanzas who needed mechanical ventilation. The universe was formed by 51 patients submitted to ventilatory support in this period. There it was detected the supremacy of the male sex and a major affectation among patients aged 50 and more years for both genres. The mortality was analyzed according to the ventilation time, dividing the patients in two groups: one of less than five days, and a second of five days or more; the biggest number of deceased was observed in the first group, with 75,7 %, while in the second group the mortality was 24,2 %. The sepsis associated or not with the ventilation, was detected in 22 patients (43,1 %); of them, 59,1 % (13 patients) was not hospital-acquired and 40,9 % (9 patients) was hospital-acquired or associated with the ventilation. The main causes that motivated the ventilation were: the post-surgical complications (39,2 %), the complicated chronic obstructive pulmonary illness (17,6 %), the cardiogenic shock (11,8 %), the hemorrhagic-type encephalo-vascular illness (11,8 %), the septic shock (9,8 %) and other causes (9,8 %). The mortality in the ventilated patients was 64,7 %, with a predominance of the surgical patients. The direct causes of decease were in correspondence with the entities that motivated the ventilation, being the most detected: the multi organic failure, the septic shock, the cardiogenic shock and the bronchopneumonia. There it was a clinic-pathologic interrelation in 93,1 % of the deceased.

Para identificar el comportamiento de los pacientes de la Unidad de Cuidados Intensivos Polivalente del Hospital Militar Mario Muñoz, de Matanzas, que necesitaron ventilación mecánica, se realizó un estudio descriptivo observacional desde enero de 2009 hasta diciembre de 2010. El universo estuvo conformado por 51 pacientes sometidos a soporte ventilatorio en este período. Se detectó una supremacía del sexo masculino y una mayor afectación a partir de los 50 años de edad para ambos sexos. Se realizó análisis de la mortalidad según el tiempo de ventilación, dividiendo los pacientes en dos grupos: uno de menos de cinco días, y un segundo de cinco días o más; observándose el mayor número de fallecidos en el primer grupo, con un 75,7 %, mientras que en el segundo grupo la mortalidad fue de 24,2 %. La sepsis, asociada o no a la ventilación, se detectó en 22 pacientes (43,1 %); de ellas el 59,1 % (13 pacientes) fueron extrahospitalarias y el 40,9 % (9 pacientes) intrahospitalarias o asociadas a la ventilación. Las principales causas que motivaron la ventilación fueron: los post quirúrgicos complicados (39,2 %), enfermedad pulmonar obstructiva crónica complicada (17,6 %), el schok cardiogénico (11,8 %), enfermedad vascular encefálica de tipo hemorrágica (11,8 %), schok séptico (9,8 %) y otras causas (9,8 %). La mortalidad en los pacientes ventilados fue de 64,7 %, con un predominio de los pacientes quirúrgicos. Las causas directas de muerte se correspondieron con las entidades que motivaron la ventilación, siendo las más detectadas: falla múltiple de órganos, schok séptico, schok cardiogénico y bronconeumonias. Existió correlación clínico patológica en el 93,1 % de los fallecidos.

We presented a female patient, aged 35 years old, with an infrequent bladder pleomorphic leiomyosarcoma of malignancy high level. She had a history of total hematuria of 7 months of evolution. We arrived to the diagnosis of the vesical tumor by citoscopy, descendent urogram and computerized axial tomography. The histological type of the tumor, a pleomorphic leiomyosarcoma, was determined by special IHQ-Alpha-Actin, specific for this kind of tumor. The surgical treatment by cystectomy was partial, followed of coadjutant chemotherapy. We described the results and the patients' evolution during 5 years. Currently the patient is tumor free, and lives her life without problems. We reviewed the literature on the theme.

Se presentó una paciente de 35 años de edad con un infrecuente leiomiosarcoma pleomórfico de vejiga de alto grado de malignidad, con una historia de hematuria total de 7 meses de evolución. El diagnóstico de tumor vesical se realizó por cistoscopia, urograma descendente y tomografía axial computarizada. El tipo histológico del tumor, leiomiosarcoma pleomórfico, se realizó por tinciones especiales de IHQ-Alfa-Actina, específica para este tipo de tumor. El tratamiento quirúrgico fue cistectomía parcial seguida de quimioterapia coadyuvante. Se describieron los resultados, y evolución de la paciente hasta los 5 años. Actualmente la paciente se encuentra libre de tumor, y mantiene una vida sin problemas. Se revisó la literatura al respecto.

Avaliaram-se as características qualitativas da carcaça de cordeiros deslanados em confinamento submetidos a dietas com dois níveis de energia. Foram utilizados 18 cordeiros de cada genótipo: Morada Nova, Santa Inês e mestiços Dorper x Santa Inês. As rações de menor e maior nível energético foram constituídas de 2,5 e 2,94 Mcal EM/kg MS, respectivamente. Foram avaliados a conformação e acabamento da carcaça, além da composição tecidual da perna. O escore para conformação foi significativamente maior nos animais mantidos com a dieta de maior nível de energia e nos mestiços Dorper x Santa Inês. Animais dos grupos Santa Inês e Dorper x Santa Inês apresentaram escore para cor da carne próximo à classe de vermelho, enquanto no genótipo Morada Nova foi próximo ao vermelho levemente escuro. A relação músculo:osso dos mestiços Dorper x Santa Inês e dos Morada Nova foi superior à observada no grupo Santa Inês. A relação músculo:gordura foi maior nos animais mantidos com a dieta de menor concentração energética. A área de olho-de-lombo dos mestiços Dorper x Santa Inês foi semelhante à do grupo Morada Nova e superior à do grupo Santa Inês. O índice de compacidade da carcaça, a espessura de gordura subcutânea e a espessura de gordura de cobertura sobre a superfície da 12ª costela foram maiores nos animais submetidos à dieta de maior nível energético. A gordura da perna foi o único tecido influenciado pela densidade calórica das dietas, enquanto o tecido ósseo sofreu influência dos genótipos, uma vez que a raça Santa Inês apresentou o maior percentual desse tecido. O genótipo e o nível de energia da dieta influenciam a conformação de carcaça. Dietas com maior densidade energética melhoram o índice de compacidade da carcaça e espessura de gordura subcutânea.

Itwas evaluated qualitative characteristics of confined hair sheep submitted to diets with two energy levels. Eighteen lambs from each genotype were used: Morada Nova, Santa Inez and Dorpper x Santa Inez crossbreds. The rations with the lowest and the highest energy level were made up with 2.5 and 2.94 Mcal EM/kg DM, respectively. It was evaluated conformation and finishing carcass, in addition to leg tissue composition. The score for conformation was significantly higher for animals kept in diet with the highest energy level and for Dorpper x Santa Inês crossbreds. Animals from Santa Inez and from Dorpper x Santa Inez breeds showed score for meat color close to red class, while Morada Nova genotype was close to slightly dark red. The muscle:bone ratio for Dorpper x Santa Inez crossbred and Morada Nova animals was higher than for Santa Inez animals. The muscle:fat ratio was higher in animals kept with the lowest energy concentration diet. The rib eye area of Dorpper x Santa Inez crossbred animals was similar to Morada Nova animals and higher than Santa Inez group. Carcass compactness index and subcutaneous fat thickness and maximum cover fat thickness on the surface of the 12th rib were higher for animals submitted to the diet with the highest energy level. Leg fat was the only tissue influenced by diet calory density, while bone tissue was affected by the genotypes, since Santa Inez breed showed the highest percentage in this tissue. The genotype and level of energy in the diet affect carcass conformation. Diets with higher energy density improve carcass compactness index and subcutaneous fat thickness.

Objetivou-se investigar a influência do genótipo e da concentração energética da dieta na qualidade química e sensorial da carne ovina. Foram avaliadas amostras provenientes de 18 animais Morada Nova, 18 Santa Inês e 18 mestiços Santa Inês x Dorper, num total de 54 animais. Os animais foram mantidos com duas dietas com concentrações energéticas de 10.46 e 12.56 MJ ME/kg e abatidos ao atingirem o peso médio de 30 kg. Foram determinados os teores de proteína, umidade, gordura e cinzas, a perda de peso por cocção, a capacidade de retenção de água, a força de cisalhamento e os atributos sensoriais sabor, dureza e suculência dessas carnes. O genótipo influenciou a composição química da carne ovina, uma vez que os animais da raça Morada Nova apresentaram o maior teor de umidade e os mestiços, o maior percentual de proteína, entretanto não houve variação nos atributos sensoriais da carne ovina desses três genótipos. A dieta com maior concentração energética proporcionou carne com maior suculência.

The objective of this study was to investigate the influence of genotype and the energy concentration in the diet on the sensorial and chemical quality of lamb meat. It was evaluated samples from 18 Morada Nova animals, 18 Santa Inez animals, and 18 Santa Inez x Dorper crossbred animals, totalizing 54 animals. The animals were kept with two diets, with energy concentrations of 10.46 and 12.56 MJ ME/kg, respectively, and slaughtered at 30 kg average weight. It was determined contents of protein, moisture, fat and ash, as well as cooking loss, water-holding capacity, shear force, and sensorial attributes of flavour, firmness and juiciness of the meat. Genotype influenced the chemical composition of lamb meat because animals of Morada Nova breed presented the highest moisture content, and Santa Inez x Dorper crossbred showed the highest protein percentage; however, there was no variation in the sensorial attributes of lamb meat of these three genotypes. The diet with the highest energy concentration provides meat with higher juiciness.

O estudo epizootiológico de Anaplasma marginale em regiões que existem vários reservatórios, co-existência de espécies de riquétsias patógenas e vetores comuns é uma tarefa complicada. Com o objetivo de obter o diagnóstico dessa riquétsia em bovinos e veado campeiro do Pantanal brasileiro foi avaliada uma reação da polimerase em cadeia em tempo real (PCR-TR) com o fluoróforo intercalante de fita dupla de DNA Sybr Green e iniciadores baseados na seqüência do gene msp5 de A. marginale comparando-a a uma PCR convencional (PCR-C) e ao exame parasitológico de esfregaço fino de sangue corado com Giemsa-MayGrunwald. Ambas PCRs apresentaram bom desempenho no diagnóstico de A. marginale nos bovinos, o qual foi superior ao exame parasitológico. O PCR-TR detectou sete veados campeiros positivos (16,3%), o que foi significativamente maior comparado ao PCR-C identificando um animal como positivo (2,3%), e ao exame parasitológico não encontrou nenhum animal positivo. A média da temperatura de dissociação das reações positivas para amostras de bovinos (81,72 ºC ± 0,20) foi idêntica àquelas dos cervídeos ( 81,72 ºC ± 0,12), o que sugere que ambas espécies animais foram infectadas por A. marginale. Concluímos que PCR-TR pode ser utilizada para diagnóstico e estudos epizootiológicos de A. marginale em bovinos e cervídeos. Apesar da pequena amostragem de veado campeiro os resultados indicam que essa espécie de animal selvagem tem importância na epizootiologia do Anaplasma no Pantanal brasileiro.

Epizootiological study of Anaplasma marginale in regions that contain various reservoir hosts, co-existence of rickettsia pathogens, and common vectors is a complicated task. To achieve diagnosis of this rickettsia in cattle and campeiro deer of Brazilian Pantanal, a comparison was made between a real time polymerase chain reaction (RT-PCR) with intercalating Sybr Green fluorochrome and primers based on msp5 gene of A. marginale; a conventional PCR (C-PCR); and parasitological examination using thin blood smear stained with Giemsa-MayGrunwald. Both PCRs showed good performance in the diagnosis of A. marginale in cattle, and were superior to the parasitological exam. The RT-PCR detected seven positive campeiro deer (16.3%). This rate was significantly higher compared to C-PCR, which identified one animal as positive (2.3%), and also compared to parasitological diagnosis, which did not find any positive animals. The dissociation temperature average of positive reactions in cattle (81.72 ºC ± 0.20) was identical to dissociation temperature found in the cervids (81.72 ºC ± 0.12), suggesting that both animal species were infected with A. marginale. We concluded that RT-PCR can be used for A. marginale diagnosis and in epizootiological studies of cattle and cervids; in spite of the small number of campeiro deer samples, the results indicated that this wildlife species has importance in the Anaplasma epizootiology in the Brazilian Pantanal.